The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration
Identifieur interne : 001575 ( Main/Exploration ); précédent : 001574; suivant : 001576The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration
Auteurs : Paco Romero [Espagne] ; Maria T. Lafuente [Espagne] ; María J. Rodrigo [Espagne]Source :
- Journal of experimental botany [ 0022-0957 ] ; 2012.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Déshydratation, Fruit, Botanique.
English descriptors
- KwdEn :
Abstract
The abscisic acid (ABA) signalling core in plants include the cytosolic ABA receptors (PYR/PYL/RCARs), the clade-A type 2C protein phosphatases (PP2CAs), and the subclass III SNF1-rotated protein kinases 2 (SnRK2s). The aim of this work was to identify these ABA perception system components in sweet orange and to determine the influence of endogenous ABA on their transcriptional regulation during fruit development and ripening, taking advantage of the comparative analysis between a wild-type and a fruit-specific ABA-deficient mutant. Transcriptional changes in the ABA signalosome during leaf dehydration were also studied. Six PYR/PYL/RCAR, five PP2CA, and two subclass III SnRK2 genes, homologous to those of Arabidopsis, were identified in the Citrus genome. The high degree of homology and conserved motifs for protein folding and for functional activity suggested that these Citrus proteins are bona fide core elements of ABA perception in orange. Opposite expression patterns of CsPYL4 and CsPYL5 and ABA accumulation were found during ripening, although there were few differences between varieties. In contrast, changes in expression of CsPP2CA genes during ripening paralleled those of ABA content and agreed with the relevant differences between wild-type and mutant fruit transcript accumulation. CsSnRK2 gene expression continuously decreased with ripening and no remarkable differences were found between cultivars. Overall, dehydration had a minor effect on CsPYR/PYL/RCAR and CsSnRK2 expression in vegetative tissue, whereas CsABI1, CsAHG1, and CsAHG3 were highly induced by water stress. The global results suggest that responsiveness to ABA changes during citrus fruit ripening, and leaf dehydration was higher in the CsPP2CA gene negative regulators than in the other ABA signalosome components.
Affiliations:
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Lafuente</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto de Agroquimica y Tecnología de Alimentos (IATA-CSIC), Consejo Superior de Investigaciones Científicas, Av. Agustin Escardino, 7</s1><s2>46980, Paterna-Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>46980, Paterna-Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Rodrigo, Maria J" sort="Rodrigo, Maria J" uniqKey="Rodrigo M" first="María J." last="Rodrigo">María J. Rodrigo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto de Agroquimica y Tecnología de Alimentos (IATA-CSIC), Consejo Superior de Investigaciones Científicas, Av. Agustin Escardino, 7</s1><s2>46980, Paterna-Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>46980, Paterna-Valencia</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">12-0363561</idno><date when="2012">2012</date><idno type="stanalyst">PASCAL 12-0363561 INIST</idno><idno type="RBID">Pascal:12-0363561</idno><idno type="wicri:Area/PascalFrancis/Corpus">000085</idno><idno type="wicri:Area/PascalFrancis/Curation">000915</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000073</idno><idno type="wicri:doubleKey">0022-0957:2012:Romero P:the:citrus:aba</idno><idno type="wicri:Area/Main/Merge">001591</idno><idno type="wicri:Area/Main/Curation">001575</idno><idno type="wicri:Area/Main/Exploration">001575</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration</title><author><name sortKey="Romero, Paco" sort="Romero, Paco" uniqKey="Romero P" first="Paco" last="Romero">Paco Romero</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto de Agroquimica y Tecnología de Alimentos (IATA-CSIC), Consejo Superior de Investigaciones Científicas, Av. Agustin Escardino, 7</s1><s2>46980, Paterna-Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>46980, Paterna-Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Lafuente, Maria T" sort="Lafuente, Maria T" uniqKey="Lafuente M" first="Maria T." last="Lafuente">Maria T. Lafuente</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto de Agroquimica y Tecnología de Alimentos (IATA-CSIC), Consejo Superior de Investigaciones Científicas, Av. Agustin Escardino, 7</s1><s2>46980, Paterna-Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>46980, Paterna-Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Rodrigo, Maria J" sort="Rodrigo, Maria J" uniqKey="Rodrigo M" first="María J." last="Rodrigo">María J. Rodrigo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto de Agroquimica y Tecnología de Alimentos (IATA-CSIC), Consejo Superior de Investigaciones Científicas, Av. Agustin Escardino, 7</s1><s2>46980, Paterna-Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>46980, Paterna-Valencia</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of experimental botany</title><title level="j" type="abbreviated">J. exp. bot.</title><idno type="ISSN">0022-0957</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of experimental botany</title><title level="j" type="abbreviated">J. exp. bot.</title><idno type="ISSN">0022-0957</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abscisic acid</term><term>Botany</term><term>Citrus sinensis</term><term>Dehydration</term><term>Fruit</term><term>Gene expression</term><term>Orange</term><term>Plant leaf</term><term>Ripening</term><term>Transcription</term><term>Transcription factor</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Transcription</term><term>Maturation</term><term>Feuille végétal</term><term>Déshydratation</term><term>Fruit</term><term>Expression génique</term><term>Botanique</term><term>Citrus sinensis</term><term>Acide abscissique</term><term>Facteur transcription</term><term>Orange</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Déshydratation</term><term>Fruit</term><term>Botanique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The abscisic acid (ABA) signalling core in plants include the cytosolic ABA receptors (PYR/PYL/RCARs), the clade-A type 2C protein phosphatases (PP2CAs), and the subclass III SNF1-rotated protein kinases 2 (SnRK2s). The aim of this work was to identify these ABA perception system components in sweet orange and to determine the influence of endogenous ABA on their transcriptional regulation during fruit development and ripening, taking advantage of the comparative analysis between a wild-type and a fruit-specific ABA-deficient mutant. Transcriptional changes in the ABA signalosome during leaf dehydration were also studied. Six PYR/PYL/RCAR, five PP2CA, and two subclass III SnRK2 genes, homologous to those of Arabidopsis, were identified in the Citrus genome. The high degree of homology and conserved motifs for protein folding and for functional activity suggested that these Citrus proteins are bona fide core elements of ABA perception in orange. Opposite expression patterns of CsPYL4 and CsPYL5 and ABA accumulation were found during ripening, although there were few differences between varieties. In contrast, changes in expression of CsPP2CA genes during ripening paralleled those of ABA content and agreed with the relevant differences between wild-type and mutant fruit transcript accumulation. CsSnRK2 gene expression continuously decreased with ripening and no remarkable differences were found between cultivars. Overall, dehydration had a minor effect on CsPYR/PYL/RCAR and CsSnRK2 expression in vegetative tissue, whereas CsABI1, CsAHG1, and CsAHG3 were highly induced by water stress. The global results suggest that responsiveness to ABA changes during citrus fruit ripening, and leaf dehydration was higher in the CsPP2CA gene negative regulators than in the other ABA signalosome components.</div></front></TEI><affiliations><list><country><li>Espagne</li></country></list><tree><country name="Espagne"><noRegion><name sortKey="Romero, Paco" sort="Romero, Paco" uniqKey="Romero P" first="Paco" last="Romero">Paco Romero</name></noRegion><name sortKey="Lafuente, Maria T" sort="Lafuente, Maria T" uniqKey="Lafuente M" first="Maria T." last="Lafuente">Maria T. Lafuente</name><name sortKey="Rodrigo, Maria J" sort="Rodrigo, Maria J" uniqKey="Rodrigo M" first="María J." last="Rodrigo">María J. Rodrigo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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